Garner uses high-resolution, single-molecule tracking and localization microscopy (PALM-based) to study the dynamic spatial organization of the bacterial cytoskeleton and cell-wall synthesis machinery in live prokaryotic cells at nanometer precision.
Hylkje Geertsema uses single-molecule super-resolution fluorescence microscopy (TIRF, SMLM, PALM/STORM) to study DNA replication dynamics. Her lab visualises and quantifies individual replication proteins at replication forks in living cells to understand the kinetics and fidelity of DNA copying. Research focuses on measuring spatiotemporal dynamics of protein assemblies during DNA metabolism with nanometre resolution.
Gooding is one of the world's most-cited biosensor scientists (inaugural editor-in-chief of ACS Sensors) and runs a group of over thirty researchers spanning surface chemistry, electrochemistry and nanomedicine. The sensing programme that matters here is the move from ensemble to digital, single-molecule-resolved detection: nanoparticle-tethered electrochemical sensors in which single binding events are counted rather than averaged, nanopore blockade sensors for protein biomarkers such as PSA, amplification-free nucleic-acid detection, and antifouling surface chemistries that make any of this work in real biological fluid. He has a strong commercialisation record (AgaMatrix glucose sensors). Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — his single-molecule-counting philosophy is the biosensing analogue of moving from a pT/sqrt(Hz) NV ensemble to single-spin detection: in both cases the sensitivity gain comes from resolving individual events rather than improving an averaged signal. He is also the obvious collaborator for anyone trying to functionalise a diamond or nanoparticle quantum sensor for a real analyte.
Gruszka's Chromatin Dynamics Lab combines real-time single-molecule imaging with biochemistry and biophysics (including in Xenopus egg-extract systems) to study how epigenetic information carried by nucleosomes is disassembled and re-established during DNA replication. The lab is actively recruiting postdoctoral fellows.
Hinde is a fluorescence-fluctuation physicist embedded in cell biology: she uses pair-correlation function analysis, number-and-brightness, phasor-FLIM and FRET to read out chromatin compaction, protein-chromatin binding dynamics and nucleocytoplasmic transport in living nuclei, at spatial and temporal scales that conventional imaging averages away. The programme is a technique-pushing one — the emphasis is on extracting nanoscale structural information from photon statistics rather than on brute-force localisation — and it is now being coupled to quantum sensing through her QUBIC investigatorship, where the goal is to combine fluorescence readouts with NV-based magnetic and spin-noise contrast in the same cell. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — her role in QUBIC is to supply the cell-biological questions and the correlative optical readouts that make pT/sqrt(Hz)-class ensemble sensing biologically interpretable. Preferred attribute present: lifetime- and orientation-resolved methods pushing past the usual resolution limits.
Hutchison works on molecular polaritonics: what happens to chemistry when molecular electronic or vibrational transitions are strongly coupled to a confined optical mode in a Fabry-Perot or plasmonic nanocavity. He was among the first to show that vibrational strong coupling modifies ground-state chemical reactivity, and the group continues to probe polariton-modified energy transfer, photochemistry and transport, alongside single-molecule spectroscopy and 2D-material photonics. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — the connection to quantum sensing is the cavity: the same Purcell and collective-coupling physics that concentrates optical density of states around a molecule is what is used to improve photon collection and readout fidelity in NV ensembles operating at pT/sqrt(Hz). This is fundamental light-matter physics with a clear nonclassical-state angle.
Jones's group develops optical tweezers instrumentation for biological applications. Research directions: (1) Single-cell mechanics — using optical traps to apply calibrated forces to cells and measure viscoelastic properties relevant to cancer invasion and immune response; (2) Motor protein biophysics — measuring force-velocity curves of kinesin/myosin motors at the single-molecule level; (3) Optical sorting — holographic optical tweezers for cell sorting by mechanical phenotype; (4) Instrument development — fast-switching AOD-based traps, quantitative phase imaging combined with force measurement. Sensitive to pN forces, combining biosensing with fundamental biophysics.
Kapanidis' Gene Machines group develops single-molecule fluorescence methods (including ALEX/FRET and super-resolution microscopy) to observe transcription and other gene-expression machinery in real time in bacteria and viruses, and leverages this toolkit to build ultrasensitive DNA-based biosensors for pathogen and antibiotic-resistance detection.
Klenerman develops and applies single-molecule fluorescence and scanning-probe methods (including nanopipette scanning ion-conductance microscopy and a single-objective oblique-plane light-sheet microscope) to study protein misfolding and aggregation in neurodegenerative disease, alongside his earlier work co-inventing next-generation DNA sequencing.
Designs programmable DNA nanodevices as quantitative fluorescent reporters to map second messengers in real time inside specific organelles of living cells. Research directions: (1) DNA origami ion-sensing nanodevices for pH, Cl-, Ca2+, HOCl, and membrane voltage with single-organelle addressability; (2) targeting nanodevices to endosomes, lysosomes, mitochondria, and ER to dissect organelle biology and disease mechanisms; (3) in vivo deployment in C. elegans and Drosophila. NIH Director's Pioneer Award 2022.