Research Areas - (7) Single-Molecule Spectroscopy

Full path: Chemistry > Physical Chemistry > Single-Molecule Spectroscopy

Department(s)/lab(s): Chemistry | Goldsmith Lab @ UWMadison
Summary:

Develops all-glass optical microresonator (microtoroid) platforms for label-free single-molecule and single-particle spectroscopy, extending single-molecule methods beyond fluorescent labels to study catalysis, protein folding, and photovoltaic materials.

Department(s)/lab(s): School of Chemistry | Hutchison Molecular Polaritonics Group @ UMelb
Summary:

Hutchison works on molecular polaritonics: what happens to chemistry when molecular electronic or vibrational transitions are strongly coupled to a confined optical mode in a Fabry-Perot or plasmonic nanocavity. He was among the first to show that vibrational strong coupling modifies ground-state chemical reactivity, and the group continues to probe polariton-modified energy transfer, photochemistry and transport, alongside single-molecule spectroscopy and 2D-material photonics. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — the connection to quantum sensing is the cavity: the same Purcell and collective-coupling physics that concentrates optical density of states around a molecule is what is used to improve photon collection and readout fidelity in NV ensembles operating at pT/sqrt(Hz). This is fundamental light-matter physics with a clear nonclassical-state angle.

Department(s)/lab(s): Chemistry | Landes Lab @ UIUC
Summary:

Develops single-molecule spectroscopy and imaging/signal-processing methods to study protein dynamics at interfaces and predictive separations.

Department(s)/lab(s): Chemistry | PPSM - Single-Molecule Photochemistry (Metivier) @ ENSPS
Summary:

Metivier (PPSM) studies photochromic and fluorescent molecules at the single-molecule level - photoswitching kinetics, energy transfer and orientation-resolved imaging - underpinning super-resolution (RESOLFT/STORM-type) probes and molecular sensors. In the broader landscape of NV-centre ensemble quantum sensing (DEER, nano-NMR, T1 relaxometry) operating near pT/sqrt(Hz) sensitivity, this work is paralleled by molecular photoswitches enabling optical super-resolution.

Department(s)/lab(s): Chemistry | Moerner Lab @ Stanford
Summary:

Nobel laureate W. E. Moerner, who first detected and studied single molecules optically, now develops engineered point-spread-function and orientation-resolved single-molecule localization microscopy methods to track individual biomolecules and their rotational dynamics in cells with nanometer precision, well beyond the optical diffraction limit.

Department(s)/lab(s): School of Chemistry | Smith Time-Resolved Spectroscopy and Microspectroscopy Group @ UMelb
Summary:

Smith runs Melbourne's time-resolved fluorescence facility and specialises in the information channels most people throw away: fluorescence lifetime, anisotropy decay and its orientational content, and single-molecule photophysics, applied to organic semiconductors, energy-transfer systems and biological samples. The group builds its own confocal microspectroscopy instrumentation for time-resolved anisotropy imaging and single-molecule detection. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — lifetime- and orientation-resolved fluorescence is the principal orthogonal contrast mechanism to spin-based sensing, and his instrumentation is the natural correlative partner for NV-ensemble DEER/relaxometry experiments at pT/sqrt(Hz) that need an independent optical readout of the same specimen. Preferred attribute present: orientation- and lifetime-resolved methods.

Department(s)/lab(s): Chemistry | Haw Yang Lab @ Princeton
Summary:

Yang's experimental physical chemistry lab designs new instrumentation to track single proteins, nanoparticles, and other emitters in three dimensions in real time within complex, heterogeneous environments, including a recent time-gated two-photon platform for high-speed 3D single-particle tracking. His group applies these single-molecule tracking and orientation-resolved imaging tools to protein conformational dynamics, functional nanostructures, and active-matter systems.