Tags - (27) biosensors

Department(s)/lab(s): EMBL Australia Node in Single Molecule Science, UNSW Medicine and Health | Sierecki Protein Interaction Networks Group @ UNSW
Summary:

Sierecki co-developed the cell-free single-molecule interaction platform with Gambin and runs a group applying it to protein interaction networks: mapping which proteins bind which, with what affinity and in what stoichiometry, at throughput high enough to screen rather than characterise one pair at a time. Recent applications include viral protein-host interactions and transcription factor complexes. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — the relevance to a quantum-sensing candidate is as a source of well-characterised, quantitatively-defined biological targets: a pT/sqrt(Hz)-class sensor is only useful in biology if someone can tell you exactly what molecular species is present and at what concentration, which is what this platform delivers. Borderline inclusion — no quantum or physics-instrumentation component — kept because single-molecule technique development is the core of the group.

Department(s)/lab(s): Electrical Engineering | Soh Lab @ Stanford
Summary:

Soh's lab engineers aptamer- and SOMAmer-based electrochemical biosensors capable of real-time, continuous molecular measurement (drugs, metabolites, proteins) directly in living systems, aiming for closed-loop, quantitative point-of-care and in vivo diagnostics.

Department(s)/lab(s): School of Chemistry | Tilley Nanomaterials and Electron Microscopy Group @ UNSW
Summary:

Tilley directs the UNSW Electron Microscope Unit and runs a nanomaterials group whose distinctive capability is in-situ liquid-cell TEM: watching nanoparticle nucleation, growth and catalytic transformation in real time inside the microscope, in liquid, rather than inferring mechanism from before-and-after snapshots. The synthetic side produces magnetic and plasmonic nanoparticles used as biosensor labels and MRI contrast agents, largely in collaboration with Gooding and Reece. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — the group is a supplier and characteriser of the nanoparticle probes that in-cell quantum sensing depends on — including the magnetic-nanoparticle labels whose stray fields a pT/sqrt(Hz) NV sensor would actually detect — and the liquid-cell TEM capability is a rare way to validate what those particles are doing in situ. Borderline inclusion (materials characterisation rather than sensing), kept for the collaborative infrastructure it represents.

Department(s)/lab(s): Genetics | Ting Lab @ Stanford
Summary:

Ting's lab invented proximity-dependent enzymatic labeling technologies (APEX, TurboID) that map the spatial proteome and transcriptome of living cells with organelle-level resolution, and develops genetically encoded fluorescent/voltage biosensors -- engineering biology's own molecular machinery into quantitative optical reporters.

Department(s)/lab(s): Electrical Engineering | Wang Lab (Spintronics/Biomagnetics) @ Stanford
Summary:

Wang develops giant-magnetoresistance (GMR) spin-valve biosensor chips that detect magnetic-nanoparticle-tagged biomolecules with high sensitivity and multiplexing for protein and nucleic-acid diagnostics -- a solid-state magnetic-sensing approach to biosensing that sits alongside NV-ensemble and OPM-based approaches at a very different sensitivity/format tradeoff.

Department(s)/lab(s): Chemistry and Chemical Biology | Whitesides Research Group @ Harvard
Summary:

Whitesides' group pioneered soft lithography and paper-based microfluidics, and has long applied these tools to low-cost point-of-care diagnostic biosensors for global health settings. Included as a borderline, not-preferred biosensing case: the sensing target (colorimetric/electrochemical assays) is compelling but device-fabrication-centric rather than a cutting-edge-sensitivity physical sensor.

Department(s)/lab(s): School of Physics / School of Chemistry | Wickham DNA Nanotechnology Group @ USyd
Summary:

Wickham builds DNA origami nanostructures — programmable, self-assembling scaffolds with nanometre-precision addressability — and uses them as molecular machines, drug-delivery vehicles and, most relevantly, as rulers and probes for single-molecule measurement. DNA origami is the standard platform for DNA-PAINT super-resolution and for positioning fluorophores, nanoparticles or spin labels at defined separations, and her group works on dynamic, reconfigurable devices that respond to biological triggers. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — DNA origami is the leading candidate technology for positioning target molecules at a controlled standoff from a near-surface NV ensemble, which is the central geometric problem in pushing NV nanoscale NMR and DEER from pT/sqrt(Hz) ensembles down to single-molecule sensitivity. Genuinely complementary skill set for a quantum-sensing candidate.