Technique - (3) DNA-PAINT super-resolution microscopy

Type: Experimental

Description: Points Accumulation for Imaging in Nanoscale Topography using transient DNA hybridization for programmable, multiplexed single-molecule localization microscopy at ~5 nm resolution.

Department(s)/lab(s): Department of Chemistry, Institute of Physical Chemistry | Dhiman Lab - Bioinspired Supramolecular Systems @ JGU
Summary:

Dhiman holds the professorship for Physical Chemistry of Supramolecular Systems at JGU and is affiliated with the Max Planck Graduate Center. Her group uses single-molecule and super-resolution fluorescence microscopy (SMLM/PAINT-type methods) to watch synthetic supramolecular polymers assemble, exchange monomers and age in real time -- i.e. applying the biological super-resolution toolkit to non-biological self-assembling matter, and toward bioinspired/adaptive systems that behave like living materials. Relative to the established NV-ensemble quantum-sensing playbook (DEER, nanoscale NMR, T1 relaxometry at pT/sqrt(Hz) ensemble sensitivity), this is a technique-driven inclusion: the emphasis is squarely on pushing spatial and temporal resolution of dye-based imaging past the ensemble limit, and it is a newer group where a postdoc would have room to shape the direction.

Department(s)/lab(s): School of Life Sciences (SV) | Schueder Lab (High-Resolution Microscopy) @ EPFL
Summary:

Schueder is a newly appointed (2025) EPFL Assistant Professor specializing in high-resolution microscopy and its biological applications. He played a key role in the development of DNA-PAINT, a super-resolution microscopy technique enabling nanometer-scale (~5 nm) visualization of cellular structures via transient programmable DNA hybridization. Research directions: (1) DNA-PAINT super-resolution — multiplexed, quantitative imaging of protein complexes in fixed and living cells with Exchange-PAINT; (2) Single-molecule localization below 5 nm resolution — resolving individual proteins within complexes; (3) Biological applications — imaging cytoskeletal networks, receptor clustering, chromatin organization; (4) Expanding to in situ structural biology — correlating super-resolution images with cryo-EM data. Transferred from ETH Zurich. Strong fit with EPFL imaging and structural biology ecosystem.

Department(s)/lab(s): School of Physics / School of Chemistry | Wickham DNA Nanotechnology Group @ USyd
Summary:

Wickham builds DNA origami nanostructures — programmable, self-assembling scaffolds with nanometre-precision addressability — and uses them as molecular machines, drug-delivery vehicles and, most relevantly, as rulers and probes for single-molecule measurement. DNA origami is the standard platform for DNA-PAINT super-resolution and for positioning fluorophores, nanoparticles or spin labels at defined separations, and her group works on dynamic, reconfigurable devices that respond to biological triggers. Positioned against the established body of NV-ensemble quantum sensing work — DEER, nanoscale NMR and T1 relaxometry protocols operating at pT/sqrt(Hz) field sensitivity — DNA origami is the leading candidate technology for positioning target molecules at a controlled standoff from a near-surface NV ensemble, which is the central geometric problem in pushing NV nanoscale NMR and DEER from pT/sqrt(Hz) ensembles down to single-molecule sensitivity. Genuinely complementary skill set for a quantum-sensing candidate.