Description: DNA/RNA labeling and detection in fixed cells using fluorescent probes.
French is Professor and former Head of the Photonics Group (2001–2013). His group at Imperial (with Dunsby and Neil) develops multidimensional fluorescence imaging technology for life sciences and clinical applications. Research portfolio: (1) FLIM — wide-field time-gated FLIM using gated optical intensifiers and TCSPC for single-cell FRET-based biosensing of protein-protein interactions, cell signalling (kinase activity), and drug-target engagement in multi-well plates; (2) Super-resolved microscopy — STED, easySTORM (lower-cost STORM), and SIM+FLIM for mapping molecular function to biological nanostructure below the diffraction limit; (3) FLIM endoscopy — flexible wide-field FLIM endoscopes for label-free cancer diagnostics (autofluorescence lifetime) and osteoarthritis cartilage; (4) Open-source imaging — automated multiwell plate FLIM reader for high-content drug screening. Satellite lab at Francis Crick Institute.
Schueder is a newly appointed (2025) EPFL Assistant Professor specializing in high-resolution microscopy and its biological applications. He played a key role in the development of DNA-PAINT, a super-resolution microscopy technique enabling nanometer-scale (~5 nm) visualization of cellular structures via transient programmable DNA hybridization. Research directions: (1) DNA-PAINT super-resolution — multiplexed, quantitative imaging of protein complexes in fixed and living cells with Exchange-PAINT; (2) Single-molecule localization below 5 nm resolution — resolving individual proteins within complexes; (3) Biological applications — imaging cytoskeletal networks, receptor clustering, chromatin organization; (4) Expanding to in situ structural biology — correlating super-resolution images with cryo-EM data. Transferred from ETH Zurich. Strong fit with EPFL imaging and structural biology ecosystem.