Technique - (1) Molecular rotor fluorescence lifetime viscosity imaging

Type: Experimental

Description: Use of viscosity-sensitive molecular rotor fluorophores combined with FLIM to map microviscosity in cells and tissue.

Marina Kuimova 🔗

Imperial College London 🔗

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Research areas:

Biology > Biophysics > FLIM / Super-Resolution Biophotonics > Molecular Rotor Viscosity FLIM Sensing

Techniques:

FLIM / metabolic imaging (NADH/FAD lifetime)
Molecular rotor fluorescence lifetime viscosity imaging

Tags:

molecular rotors viscosity sensing
photodynamic therapy

Department(s)/lab(s): Chemistry | Kuimova Research Group @ Imperial

Summary:

Kuimova pioneered the use of fluorescent 'molecular rotor' probes combined with fluorescence lifetime imaging (FLIM) to quantitatively map intracellular microviscosity in live cells and tissue, with applications spanning photodynamic therapy, membrane biophysics and G-quadruplex DNA imaging.