Allemand co-pioneered single-molecule magnetic-tweezer manipulation of DNA and RNA, using calibrated magnetic forces/torques to measure the torsional and stretching mechanics of nucleic acids and the real-time kinetics of the motor proteins (helicases, polymerases, topoisomerases) that act on them. His joint lab with Vincent Croquette continues to develop new magnetic-tweezer instrumentation (including high-throughput and torque-sensing variants) applied to DNA replication, repair, and RNA processing machinery.