Research Areas - (2) Single-Molecule RNA Dynamics and CRISPR Sensing

Full path: Biology > Biophysics > Quantum Biology / Biosensing > Single-Molecule RNA Dynamics and CRISPR Sensing

Chirlmin Joo 🔗
Delft University of Technology 🔗
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Department(s)/lab(s): BioNanoscience / Kavli Institute of Nanoscience | Chirlmin Joo Lab — Single-Molecule RNA and CRISPR @ TU Delft
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Chirlmin Joo (Full Professor, BioNanoscience) uses single-molecule fluorescence to study RNA dynamics and CRISPR-Cas. Research: (1) single-molecule FRET and direct RNA imaging — visualizing RNA folding, ribozyme catalysis, and mRNA translation dynamics; (2) CRISPR-Cas mechanism — real-time observation of Cas9 and Cas13 target search and cleavage; (3) nanopore-based protein sensing integration with optical tools. ERC Grant.

David Rueda 🔗
Imperial College London 🔗 MRC London Institute of Medical Sciences 🔗
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Department(s)/lab(s): Medicine | Rueda Single-Molecule Imaging Group @ Imperial
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Rueda leads a single-molecule imaging group (jointly at Imperial and the MRC London Institute of Medical Sciences) that combines single-molecule FRET, fluorogenic RNA aptamer imaging and optical tweezers to reveal the structural dynamics of RNA folding/splicing, CRISPR-Cas9 target search and off-target activity, and chromatin-remodelling complexes; the aptamer-imaging technology has been spun out as the startup Irida.